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MGIEasy RNA Library Prep Set

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Description

MGIEasy RNA Library Prep Set provides an efficient solution for generating libraries from a wide range (10 ng-1 ug) of total RNA that is suitable for MGI high-throughput sequencing platforms. It is easy to use and can be widely applied to human, animal, plant and microbe transcriptome research for comprehensive and rapid analysis of gene expression changes, examining rare and novel transcripts, the discovery of alternative splicing events, gene fusions, SNPs and allele-specific expression.

Features

  1. Wide range of total RNA input
    Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
  2. Compatible with various sample types
    Including low-quality RNA, FFPE RNA, and plasma samples
  3. Excellent coverage
    Accurate and comprehensive mapping of transcripts
  4. High uniformity
    Enables detection of complete transcripts without bias at either end
  5. Easy to Use
    Simple protocol and short operation time, suitable for automated library preparation instrument
  6. Flexible Solutions
    Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types

Applications

Sample type RNA enrichment method Read length Applications
Eukaryotic total RNA with high integrity Poly(A)+ mRNA enriched by oligo(dT) beads SE50/ PE100/ PE150 mRNA quantification and transcriptome analysisMRNA
Degraded RNA from FFPE samples or plasma cell-free RNA etc. rRNA depleted with rRNA depletion kit SE50/ PE100 RNA quantification and transcriptome analysis

Specification

Product name: MGIEasy RNA Library Prep Set
Version: V3.0
Reaction/Set: 16 RXN (1000006383) 96 RXN (1000006384)
Storage temperature requirement: -18℃~-25℃
Input Quantity: 10 ng-1 ug of total RNA
Sample types: tissues, plasma and FFPE sample
Species Compatibility Human, animals, plants, fungi and bacteria such as, mouse, rice, Arabidopsis, yeast and E.coli.
Assay Time: 7 h
Applications: RNA-Seq, Transcriptome Sequencing
Platform Compatibility: BGISEQ-500, MGISEQ-2000
Recommended Read Length: SE50/PE100/PE150
Recommended sequencing data per sample: 25 M raw reads(SE50)/8 Gb raw data(PE100/PE150)

Files

Data Sheet MGIEasy RNA Directional Library Pre Set Download
User Manual:

1000006383, 1000006384-MGIEasy RNA Library Prep Kit User Manual Download

This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.

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Demo Data

Read Length: PE150 Sample: UHRR Platform: MGISEQ-2000

I Sample Information Fastq Download
II Sample Information Fastq Download
III Sample Information Fastq Download
Ⅳ Sample Information Fastq Download